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Expression and <t>subcellular</t> localization analysis of the OsU2AF65B gene in O. sativa . ( A ) Heatmap of OsU2AF65B gene expression ( LOC_Os11g45590.2 ) in shoots and roots (log 2 -transformed data). Experiments were repeated at least three times. ( B ) Subcellular localization of OsU2AF65B. Rice protoplasts were transfected with OsU2AF65B-PGREENII-UBI-GFP and PGREENII-UBI-GFP. Green fluorescence represents the expression site of the fusion protein. Scale bar = 10 μm.
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Expression and <t>subcellular</t> localization analysis of the OsU2AF65B gene in O. sativa . ( A ) Heatmap of OsU2AF65B gene expression ( LOC_Os11g45590.2 ) in shoots and roots (log 2 -transformed data). Experiments were repeated at least three times. ( B ) Subcellular localization of OsU2AF65B. Rice protoplasts were transfected with OsU2AF65B-PGREENII-UBI-GFP and PGREENII-UBI-GFP. Green fluorescence represents the expression site of the fusion protein. Scale bar = 10 μm.
Wolf Psort, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Expression and <t>subcellular</t> localization analysis of the OsU2AF65B gene in O. sativa . ( A ) Heatmap of OsU2AF65B gene expression ( LOC_Os11g45590.2 ) in shoots and roots (log 2 -transformed data). Experiments were repeated at least three times. ( B ) Subcellular localization of OsU2AF65B. Rice protoplasts were transfected with OsU2AF65B-PGREENII-UBI-GFP and PGREENII-UBI-GFP. Green fluorescence represents the expression site of the fusion protein. Scale bar = 10 μm.
Wolfpsort, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Expression and <t>subcellular</t> localization analysis of the OsU2AF65B gene in O. sativa . ( A ) Heatmap of OsU2AF65B gene expression ( LOC_Os11g45590.2 ) in shoots and roots (log 2 -transformed data). Experiments were repeated at least three times. ( B ) Subcellular localization of OsU2AF65B. Rice protoplasts were transfected with OsU2AF65B-PGREENII-UBI-GFP and PGREENII-UBI-GFP. Green fluorescence represents the expression site of the fusion protein. Scale bar = 10 μm.
Wolepsort Online Tool, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Expression and <t>subcellular</t> localization analysis of the OsU2AF65B gene in O. sativa . ( A ) Heatmap of OsU2AF65B gene expression ( LOC_Os11g45590.2 ) in shoots and roots (log 2 -transformed data). Experiments were repeated at least three times. ( B ) Subcellular localization of OsU2AF65B. Rice protoplasts were transfected with OsU2AF65B-PGREENII-UBI-GFP and PGREENII-UBI-GFP. Green fluorescence represents the expression site of the fusion protein. Scale bar = 10 μm.
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Expression and <t>subcellular</t> localization analysis of the OsU2AF65B gene in O. sativa . ( A ) Heatmap of OsU2AF65B gene expression ( LOC_Os11g45590.2 ) in shoots and roots (log 2 -transformed data). Experiments were repeated at least three times. ( B ) Subcellular localization of OsU2AF65B. Rice protoplasts were transfected with OsU2AF65B-PGREENII-UBI-GFP and PGREENII-UBI-GFP. Green fluorescence represents the expression site of the fusion protein. Scale bar = 10 μm.
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Expression and <t>subcellular</t> localization analysis of the OsU2AF65B gene in O. sativa . ( A ) Heatmap of OsU2AF65B gene expression ( LOC_Os11g45590.2 ) in shoots and roots (log 2 -transformed data). Experiments were repeated at least three times. ( B ) Subcellular localization of OsU2AF65B. Rice protoplasts were transfected with OsU2AF65B-PGREENII-UBI-GFP and PGREENII-UBI-GFP. Green fluorescence represents the expression site of the fusion protein. Scale bar = 10 μm.
Online Software Wolf Psort Ii, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Expression and subcellular localization analysis of the OsU2AF65B gene in O. sativa . ( A ) Heatmap of OsU2AF65B gene expression ( LOC_Os11g45590.2 ) in shoots and roots (log 2 -transformed data). Experiments were repeated at least three times. ( B ) Subcellular localization of OsU2AF65B. Rice protoplasts were transfected with OsU2AF65B-PGREENII-UBI-GFP and PGREENII-UBI-GFP. Green fluorescence represents the expression site of the fusion protein. Scale bar = 10 μm.

Journal: International Journal of Molecular Sciences

Article Title: A Comprehensive Analysis of the Alternative Splicing Co-Factor U2AF65B Gene Family Reveals Its Role in Stress Responses and Root Development

doi: 10.3390/ijms26083901

Figure Lengend Snippet: Expression and subcellular localization analysis of the OsU2AF65B gene in O. sativa . ( A ) Heatmap of OsU2AF65B gene expression ( LOC_Os11g45590.2 ) in shoots and roots (log 2 -transformed data). Experiments were repeated at least three times. ( B ) Subcellular localization of OsU2AF65B. Rice protoplasts were transfected with OsU2AF65B-PGREENII-UBI-GFP and PGREENII-UBI-GFP. Green fluorescence represents the expression site of the fusion protein. Scale bar = 10 μm.

Article Snippet: The GenScript Protein Subcellular Localization Prediction Tool (PSORT II) ( https://www.genscript.com/psort.html ) (accessed on 23 September 2022) was employed to predict the expression sites of U2AF65B proteins in representative plant species.

Techniques: Expressing, Gene Expression, Transformation Assay, Transfection, Fluorescence